プロジェクトのご紹介 7 - Project 7 -
シュワン細胞のダイレクト・リプログラミングと末梢神経損傷の再生
Direct reprogramming of functional Schwann cells that promote regeneration of peripheral nerve
Schwann cells (SCs) play pivotal roles in the maintenance and regeneration of the peripheral nervous system.Although transplantation of SCs enhances repair of experimentally damaged peripheral and central nerve tissues, it is difficult to prepare a sufficient number of functional SCs for transplantation therapy without causing adverse events for the donor. Here, we generated functional SCs by somatic cell reprogramming procedures and demonstrated their capability to promote peripheral nerve regeneration. Normal human fibroblasts were phenotypically converted into SCs by transducing SOX10 and Krox20 genes followed by culturing for 10 days resulting in approximately 43% directly converted Schwann cells (dSCs). The dSCs expressed SC-specific proteins, secreted neurotrophic factors, and induced neuronal cells to extend neurites. The dSCs also displayed myelin-forming capability both in vitro and in vivo. Moreover, transplantation of the dSCs into the transected sciatic nerve in mice resulted in significantly accelerated regeneration of the nerve and in improved motor function at a level comparable to that with transplantation of the SCs obtained from a peripheral nerve. The dSCs induced by our procedure may be applicable for novel regeneration therapy for not only peripheral nerve injury but also for central nerve damage and for neurodegenerative disorders related to SC dysfunction. (Sowa et al, Stem Cells Transl Med.).
一部プロジェクトのご紹介 - See some projects among others -
- 新型コロナウイルス・オミクロン変異株に対する茶カテキン類の効果
Effects of tea catechin-related compounds on Omicron subvariants of SARS-CoV-2 - 腫瘍微小環境の低pHはプロトンセンサーGたんぱく共役レセプターをを介して腫瘍のPDL-1の発現を低下させる
Extracellular acidity in tumor tissue upregulates programmed cell death protein 1 expression on tumor cells via proton-sensing G protein-coupled receptors - 緑茶カテキン、カテキン誘導体、およびガレート型テアフラビンによる新型コロナウイルスの試験管内での有意な不活化；紅茶と緑茶による試験管内での唾液中の新型コロナウイルスの迅速な不活化
Significant Inactivation of SARS-CoV-2 by a Green Tea Catechin, a Catechin-derivative and Galloylated Theaflavins in vitro; Rapid Inactivation in vitro of SARS-CoV-2 in Saliva by Black Tea and Green Tea - 新規多孔性３Dスキャフォールドと直接誘導骨芽細胞を用いた骨再生
Nanogel tectonic porous 3D scaffold for direct reprogramming fibroblasts into osteoblasts and bone regeneration - TGFR阻害剤による骨芽細胞のケミカル・ダイレクト・リプログラミング
Chemical direct reprogramming of human fibroblasts into osteoblasts by a TGFR blockade - 筋芽細胞のダイレクト・リプログラミングと筋管形成
Direct reprogramming of functional myoblasts that are capable of forming multinuclear myotube - シュワン細胞のダイレクト・リプログラミングと末梢神経損傷の再生
Direct reprogramming of functional Schwann cells that promote regeneration of peripheral nerve - 褐色脂肪のダイレクト・リプログラミングと代謝疾患の制御
Direct reprogramming of functional brown adipocytes that control metabolic diseases - 骨芽細胞のダイレクト・リプログラミングと骨再生
Direct reprogramming of functional osteoblasts that regenerate bone tissue - IL-21 によるアレルギー応答の制御とその分子機構
IL-21-mediated regulation of allergic responses and its molecular mechanisms - IL-27 による NK 細胞活性化と ADCC を介した腫瘍抑制
IL-27 augments NK cytotoxicity and induces ADCC-based tumor suppression - EBV- エピゾーマルベクターによる外来遺伝子の高効率導入、高発現、長期間持続の機構解明
Mechanisms underlying the high-rate transfection, high-level expression, and long-term maintenance of exogenous genes mediated by the Epstein-Barr virus-based episomal vector - 細胞の分化と初期化に伴う染色体エピジェネティック修飾の解析
Analyses of epigenetic modification of chromosomes upon cell differentiation and reprogramming